Abbott Alinity I, Manual Pre-treatment precipitation. Chemiluminescent Microparticle Immunoassay (CMIA)

Whole Blood: EDTA

2-8℃: 7 days      

Consensus document: therapeutic monitoring of tacrolimus (FK-206). Ther Drug Monit 995; 17(6): 606 - 14.

24 Hours

ug/L

Target 24hr trough levels: 5 - 20

‍

Histology Processing. Diagnosis.

*Any unstained slide cases for staining only must be accompanied by

• HLF64 Blocks and Slides Chain of Custody Log or, an approved local version
• Clear identification of case number on slide

*Any block cases for microtomy and staining only must be accompanied by

• HLF64 Blocks and Slides Chain of Custody Log or, an approved local version
• Clear identification of case number on block

*Any block cases for microtomy, staining and reporting should be accompanied by

• HLF64 Blocks and Slides Chain of Custody Log or, an approved local version, and a Test Request Form with gross description
• Previous history where available

Any slide cases for reporting only should be accompanied by the following:

• HLF16 Chain of Custody Log or an approved local version
• A copy of gross report and test request form
• Previous history where available
• Clear identification of case number on slide

Slides, and specimen tissue blocks are both stable at room temperature. They should be kept out of direct sunlight.

Slides should be securely placed and sealed in a slide mailer or slide tray.

Specimen tissue blocks should be wrapped in a piece of tissue.

Clients may submit tissue blocks for microtomy and/or staining only to Eurofins Pathology. Clients may submit unstained slides for staining only.

Clients may submit pathology material for Consultant Services. In some instances, routine histology technical work up may also be carried out in Eurofins Pathology prior to submission to a Consultant Pathologist.

As per service level agreement with client.

Abbott Alinity i Chemiluminescent Microparticle Immunoassay (CMIA)

Serum: Serum, Serum separator

Plasma: Dipotassium EDTA

20-25℃: 8 hrs            

2-8℃: 7 days      

-20℃: longer

Abbott IFU

Testosterone regulates spermatogenesis in males via Sertoli cell stimulation and maintenance of the HPG axis. In females, it contributes to follicular development and ovarian function. Dysregulated levels can impair gametogenesis, ovulatory function, and fertility outcomes in both sexes.

24 Hours

nmol/L

Male 21 - 49 years: 8.33 - 30.19

Male >50 years: 7.66 - 24.82

Abbott Alinity c, Enzyme Immunoassay

Serum: Serum tubes (with or without gel barrier)

2-8°C: 3 month

-20°C: 3 month

Abbott IFU

24 Hours

mg/L

Adult: 8 - 20

Abbott Alinity. CMIA

Serum (Gold and red cap)

Temperature: + 4ºC

Serum: RT 24 hours,

2-8ºC 3 days.

30 days @ -20ºC

Abbott IFU

TRAb is a third generation assay to detect anti-TSH receptor antibody using the human thyroid-stimulating monoclonal antibody M22. The M22 antibody acridinium-labelled conjugate competes with TRAb in the specimen for the bound human TSH receptor on the microparticle. Elevations of these thyrotropin receptor antibodies demonstrated high clinical sensitivity and specificity for Graves' disease when used as an aid in the differential diagnosis and etiology of hyperthyroidism. These antibodies can be classified as stimulating, inhibitory, or neutral; and the stimulating type of antibody is not subject to the typical negative feedback of elevated thyroid hormone levels. This results in continuous stimulation of the thyroid leading to the typical thyrotoxic symptoms of Graves' hyperthyroidism.

Preparation of Patient: No special preparation.

4 working days

Negative: < 3.1 IU/L

Positive: ≥ 3.1 IU/L

Abbott Alinity i Chemiluminescent Microparticle Immunoassay (CMIA)

Serum: Serum, Serum separator

Plasma: Potassium EDTA, Sodium heparin, Lithium heparin

2-8℃: 7 days      

-20℃: 6 months

Pediatric: CALIPER

Adult: Abbott IFU

TSH, secreted by the anterior pituitary, stimulates T3 and T4 production and is regulated by thyrotropin-releasing hormone (TRH). Abnormal levels may indicate thyroid dysfunction, which can disrupt menstrual cycles, ovulation, and implantation, impacting fertility.

Ref: https://pmc.ncbi.nlm.nih.gov/articles/PMC10298303/

24 Hours

mIU/L

Pediatric:  

4 day to  < 6 months: 0.73 - 4.77    

6 months  to < 14 year: 0.70 - 4.17    

14 to  < 19 year:  0.47 - 3.41          

‍

Adult:      

19 - 120  year:  0.35 - 4.94

Abbott Alinity i Chemiluminescent Microparticle Immunoassay (CMIA)

Serum: Serum, Serum separator

Plasma: Potassium EDTA, Lithium heparin, Lithium heparin plasma separator, Sodium heparin

2-8℃: 6 days        

-20℃: 6 days

Abbott IFU

24 hours

nmol/L

Adult: 63 - 151

CALCULATION based on Abbott Alinity methodologies for  Iron and UIBC (Latent capacity)

Serum: Serum tubes (with or without gel barrier)

Plasma Collection tubes

Acceptable anticoagulants are: Lithium heparin (with or without gel barrier) Sodium heparin

20-25℃: 7 days                  

2-8℃: 7 days      

-20℃: 1 year

Abbott IFU

24 Hours

Adult: 44.8 - 76.1

Abbott Alinity i Chemiluminescent Microparticle Immunoassay (CMIA)

Serum: Serum, Serum separator

2-8℃: 1 day 

-20℃: 24 weeks

NCCP Prostate Cancer GP Referral Guideline v 5 2018

24 Hours

ng/mL

Adult Male <50 years: < 2.0

Adult Male 50 - 59 years: < 3.0

Adult Male 60 - 69 years: < 4.0

Adult Male >70 years: < 5.0

Abbott Alinity c, Biuret Reaction

Serum: Serum Serum separator

Plasma Dipotassium EDTA Lithium heparin Lithium heparin separator Sodium heparin

20-25°C: 7 days

2-8°C: 7 days

-20°C: 3 months

Abbott IFU

24 Hours

g/L

Adult, ambulatory: 64 - 83

Abbott Alinity c, Immuno turbidimetric

Serum: Serum tubes (with or without gel barrier)

Plasma Collection tubes Acceptable anticoagulants are: Lithium heparin (with or without gel barrier) Sodium heparin EDTA

20-25°C: 3 days

2-8°C: 3 days

-20°C: 6 months

Abbott IFU

24 Hours

g/L

Male 14 to 60 Years: 1.74 - 3.64

Female 14 to 60 Years: 1.80 - 3.64

Male 60 to 80 Years: 1.63 - 3.44

Female 60 to 80 Years: 1.73 - 3.60

CALCULATION based on Abbott Alinity methodologies for  Iron and UIBC (Latent capacity)

Serum: Serum tubes (with or without gel barrier)

Plasma - Acceptable anticoagulants are: Sodium heparin Potassium EDTA Sodium citrate

20-25℃: 7 days                

2-8℃: 7 days      

-20℃: 1 year

Abbott IFU

24 Hours

%

Male Adult: 20.0 - 50.0

Female Adult: 15.0 -50.0

Nal Von Minden GmbH- Point of Care dipstick

Urine Clean plastic or glass container

2-8℃: 2 days        

-20℃: longer

Nal Von Minden GmbH IFU

24 Hours

ng/ml

Positivity Cut-off: 1000

Abbott Alinity c, Glycerinphosphate oxidase

Serum: Serum separator

Plasma: Lithium heparin Lithium heparin separator Sodium heparin

20-25℃: 2 days                

2-8℃: 7 days      

-20℃: 3 months

European Guidelines. http://www.eas-society.org/guidelines-2.aspx

24 Hours

mmol/L

Adult (ideal, fasting): 0.6 - 1.7

Abbott Alinity i Chemiluminescent Microparticle Immunoassay (CMIA)

Serum:  Serum with and without separator Serum with thrombin-based clot activator

Plasma: Lithium heparin with and without separator K2-EDTA K3-EDTA

20-25℃: 8 hrs            

2-8℃: 24 hours                

-20℃: 31 days

Abbott IFu

24 Hours

pg/mL

Male: < 34

Female: < 16

Fluoroenzyme immunoassay (Phadia 250)

‍

Tube Type: Serum (Gold and red cap);

Temperature: RT for up to 2days. Store at 2°C to 8°C for up to one week, or else at –20°C

‍

Serum:

RT for up to 2days. Store at 2°C to 8°C for up to one week, or else at –20°C

ImmunoCAP Tryptase DFU

4 working days

‍

ug/L

‍

(<11.4)

An internal Phadia Thermofisher study with 163 self-reported healthy men and women, aged 0-68 years, was conducted.

The ImmunoCAP Tryptase results obtained are as follows:

- Geometric mean = 3.3 μg/l

- 95th percentile = 8.4 μg/l

‍

Abbott Alinity c, Ferene

Serum: Serum tubes (with or without gel barrier)

Plasma Collection tubes Acceptable anticoagulants are: Lithium heparin (with or without gel barrier) Sodium heparin

20-25°C: 7 days

2-8°C: 3 weeks

-20°C: 1 year

Abbott IFU

24 Hours

µmo/L

Male: 12.4 - 43.0

Female: 12.5 - 55.5

Abbott Alinity c, Urease

Serum: Serum tubes (with or without gel barrier)

Plasma Collection tubes Acceptable anticoagulants are: Lithium heparin (with or without gel barrier) Sodium heparin

20-25℃: 7 days                  

2-8℃: 7 days      

-20℃: 1 year

Abbott IFu

24 Hours

mmol/L

Adult, Male <50years: 3.2 - 7.4

Adult, Female <50years: 2.5 - 6.7

Adult Male >50years: 3.0 - 9.2

Adult Female >50years: 3.5 - 7.2

Abbott Alinity c, Uricase

Serum: Serum tubes

Serum separator tubes

Plasma Lithium heparin tubes

Lithium heparin separator tubes

Sodium heparin tubes

20-25°C: 8 hour

2-8°C: 3 days

-20°C: 3 months

Abbott IFU

24 Hours

mmol/L

Male 13-79 years: 220 - 450

Female 13-79 years: 150 - 370

Abbott Alinity c, Uricase

Urine (24 hour) Clean plastic or with or without  preservatives.

OR

Urine (random specimens or timed specimens collected over intervals shorter than 24 hours) Clean plastic or glass container with or without preservatives.

20-25°C: 7 days

2-8°C: 7 days

-20°C: 1 year

Abbott IFu

24 Hours

Urine (24hr): umol/ 24hr

Urine (Random): umol / L

Male (Purine Free Diet): <2480

Female (Purine Free Diet): slighly lower

Male (Low Purine Diet): < 2830

Female (Low Purine Diet): < 2360

High Purine Diet: < 5900

Average Diet: 1480 - 4430

Abbott Alinity c, Enzymatic

Urine Clean plastic or glass container

20-25℃: 2 days                

2-8℃: 6 days        

-20℃: 6 months

EWDTS guidelines 2004

24 Hours

mmol/L

Normally concentrated urine: > 2.0

Dilute urine sample: 0.5 - 2.0

Sample integrity questionable: < 0.5

Abbott Alinity c, DRI pH-Detect Test

Urine Clean plastic or glass container

2-8℃: 5 days

DRI pH-Detect test IFU

24 Hours

Normal: 4.7 - 7.8

CALCULATION based on Abbott Alinity methodologies for urinary protein and urinary creatinine

Urine (24hr/timed). Clean plastic or glass container without preservatives.

20-25℃: 2 days                

2-8℃: 6 days        

-20℃: 6 months

Abbott IFU

24 Hours

20 ml - Spot urine sample

Refrigerated

17 days

<1.00 ug/g Creat

Urine Sample

Refrigerated

17 days

Reference values:

General population: < 0.65 μg/L *

General population: < 0.54 μg/g creatinine

Limit of quantification = 0.25 μg/L

Note: * Biological Interpretation Values (BIV) from the general adult population:

Urinary chromium: 0.65 μg/L (0.54 μg/g creatinine) (95th percentile) (VBR ANSES, 2017) [Anses, 2017].

Biological Interpretation Values (BIV) for the workplace: - French VBI (VLB) :

For exposure to chromium VI in the chromium plating sector only: Urinary chromium: 2.5 μg/L

(1.8 μg/g. creatinine) at the end of the shift and on weekends (VLB ANSES, 2017) [Anses, 2017]

- ACGIH American VBI (BEI):

Total urinary chromium: 0.7 μg/L at the end of shifts and weekends (ACGIH, 2020)

1 tube : Urine -24 hours collection

‍

Refrigerated

‍

6 days

Normal: less than 0.8 umol/24hr (50 ug/24h)

Cholestasis, hepatic cirrosis, covert wilsons disease: greater

than 0.8 umol/24h (50 ug/24h)

Frank Wilsons disease : greater than 1.6 umol/24h (100 ug/24h)

‍

Manual Dipstick - Siemens Multi-Stix

MSU/CSU. minimum 5 mL. In an appropriate sterile leak proof container.

Temperature: 2-8°C

2 days @ 2-8ºC

Urine dipsticks can test a sample for protein, blood, leucocytes, nitrite, glucose, ketone, pH, specific gravity, bilirubin and urobilinogen.

PLEASE NOTE: Samples and accompanying relevant patient/ isolate data maybe referred for confirmatory or further laboratory testing to Reference laboratories. Relevant Public Health departments may also be notified IF a notifiable disease is identified under the Infectious Diseases Regulations https://www.hpsc.ie/notifiablediseases/listofnotifiablediseases/

24 hours

*Excludes Sundays and Bank Holidays

Glucose: Negative

Bilirubin: Negative

Ketones: Negative

Specific Gravity: 1.001-1.035

Blood: Negative

pH: 4.6-8.0

Protein: Negative

Urobilinogen: <=16umol/L

Nitrite: Negative

Leukocytes: Negative

SIEMENS Multistix Healthcare Diagnostics Reagent Strips for Urinalysis TN30152B Rev. 01/19

End of working shift - Spot Urine or 24-hour

urine sample

2 tubes

‍

Refrigerated

‍

17 days

‍

<150 ug/g creat

‍

2 mL : Urine sample

‍

Refrigerated

‍

17 days

‍

Reference values Urine :

General population: < 4.6 μg/g of creatinine

Limit of quantification : = 0.5 μg/L

Biological Interpretation Values (BIV) from the general population:

3.3 μg/L (4.6 μg/g creatinine) (95th percentile in adults in the

general population aged 18 to 74), Esteban study 2014-2016 |

Biological Interpretation Values (BIV) for the workplace :

-European VBI (BLV) :

For exposure to elemental mercury and inorganic divalent compounds: Urinary mercury: 30 μg/g creatinine [SCOEL, 2007 -ACGIH American VBI (BEI) :

For exposure to metallic and inorganic mercury: Urinary mercury: 20 μg/g creatinine before the shift (ACGIH, 2013)[G1].

‍

2 mL : Urine sample

‍

Refrigerated

‍

17 days

‍

General population: < 3.8 μg/g of creatinine

Limit of detection: 1.0 ug/L

‍

Abbott Alinity c, Benzethonium chloride

Urine (24hr/timed). Clean plastic or glass container without preservatives.

20-25°C: 1 day

2-8°C: 7 days

-20°C: 1 month

Abbott IFU

24 Hours

mg/L

Adult: 0 - 300

Abbott Alinity c, Particle-enhanced turbidimetric inhibition immunoassay (PETINIA)

Serum: Serum tubes (with or without gel barrier)    

Plasma-Acceptable anticoagulants:    

Lithium heparin, Sodium heparin    

Potassium EDTA. Heparin gel plasmsa separator

2-8℃: 2 days      

-20℃: 7 days

Abbott IFU

24 Hours

µg/mL

Adult: 50 - 100

Abbott Alinity c, Homogeneous particle enhanced turbidimetric inhibition immunoassay (PETINIA)

Serum: Serum tubes (with or without gel barrier)

Plasma: Acceptable anticoagulants are: Sodium heparin, Lithium heparin K2-EDTA, K3-EDTA

2-8°C: 7 days

-10°C: 14 days

Therapeutic monitoring of vancomycin in adult patients

24 Hours

µg/mL

Trough: 5 - 20

Peak: 20 - 40

Abbott Alinity i Chemiluminescent Microparticle Immunoassay (CMIA)

Serum: Serum, Serum separator.  

Plasma: Lithium heparin plasma separator, Sodium heparin, Dipotassium EDTA

20-25℃: 3 days                

2-8℃: 7 days      

-20℃: longer

Abbott IFU

24 Hours

pg/mL

Adult: 200-883

Abbott Alinity i Chemiluminescent Microparticle Immunoassay (CMIA)

Serum: Serum, Serum separator

Plasma: Dipotassium EDTA, Tri potassium  EDTA Sodium heparin Lithium heparin powder Lithium heparin plasma separator

20-25℃: 72 hrs                

2-8℃: 12 days      

-20℃: 1 year

1. Dietary reference intakes for calcium and Vitamin D. Washington, DC: The National Academies Press. 2. J Clin Endocrinol Metab, October 2011, 96(10):2987- 2996.

The 25-hydroxyvitamin D, 25(OH)D, test accurately reflects the concentration of vitamin D.  Vitamin D is composed of vitamin D2 and vitamin D3. Individual testing for vitamin D2 and D3 may be useful as follow-up testing in individuals who do not respond to therapeutic vitamin D supplementation.

The HSE sets a budget for diagnostic tests, ordered by GP's, under the Public Spending Code (PSC).  Testing for Vitamin D deficiency is only recommended when a patient presents with clinical indications such as osteoporosis, rickets, malabsorption, raised PTH, H/L Phosphate, Pagets etc. Otherwise, the HSE recommend supplementation of Vitamin D from October to May when the sun is not strong enough in Ireland to make Vitamin D.

Patients may wish to pay for a private Vitamin D test with their GP.

24 Hours

nmol/L

Adult: 30 - 125

‍

25-OH-VITAMIN D CUTOFFS:

Increased risk of deficiency: < 30

Increased risk of in-adequacy: < 40

Adequacy: > 50

Increased risk of excess: > 125

‍

Genomic DNA extraction and purification. • Multiplex PCR amplification of the STS markers corresponding to the three AZF loci involved in the deletions (AZFa, AZFb and AZFc) on the Y chromosome. • Determination of the presence or absence of the analyzed markers, by means of agarose gel electrophoresis (according to PMID: 37674303)

‍

4mls Whole blood K3 EDTA.

(Refrigerate the sample)

‍

4ºC: 1 Months

14 Days

‍

2ml

Serum

Sodium Heparin Plasma Lithium Heparin

Plasma

No gel separator tubes

‍

Refrigerated

‍

6 days

‍

Normal range for all ages : 10.7 - 24.5 umol/L

May have no clinical significance: 7.7 - 10.7

Deficiency may be indicated : <7.7

High Zinc levels are usually caused by increased intake or sample contamination.

Zinc levels are affected by acute phase reactions, certain drugs and pregnancy. Concomitant measurement of C-reactive protein may be useful as an aid to interpreting low zinc concentration.

Other causes of low zinc levels include:

1. Decreased intake: restrictive diets, parenteral nutrition, lack of absorption (GIT and bariatric surgery, celiac disease, IBD)
2. Transport deficiency (hypoalbuminaemia especially secondary to liver cirrhosis)
3. Increased elimination (diarrhoea)
4. Genetic causes (very rare)

Abbott Alinity i Chemiluminescent Microparticle Immunoassay (CMIA)

Serum:

Serum, Serum separator

Plasma:

Dipotassium EDTA, Tripotassium EDTA

Lithium heparin

Lithium heparin plasma separator

Sodium heparin

2-8℃: 7 days      

-20℃: 1 year

Abbott IFU

24 hours

Abbott: mIU/mL

Roche: l IU/L

Males and Non-Pregnant Females: < 5.0

Pregnancy- Weeks LMP:

1-10: 202 - 231000

11- 15: 22536 - 234990

16 - 22: 8007 - 50064

23 - 40: 1 600 - 49 413